waa maxay sababta nitrogen dareere ah loogu isticmaalo ilaalinta cryopreservation?
1. Maxaad u isticmaashaa nitrogen dareere qaboojiye ahaan?
1. Sababtoo ah heerkulkanitrogen dareere ahlafteedu aad bay u hoosaysaa, laakiin dabeecaddeedu aad bay u khafiifsan tahay, wayna ku adag tahay in nitrogen dareeraha ahi ay ka soo baxdo fal-celin kiimikaad, sidaa awgeed waxa inta badan loo isticmaalaa qaboojiye ahaan.
2.nitrogen dareere ahuumi si uu u nuugo kulaylka, hoos u dhigo heerkulka, waxaana loo isticmaali karaa qaboojiye ahaan.
3. Guud ahaan, ammonia waxaa loo isticmaalaa qaboojiye ahaan iyo biyaha sida nuuga.
4. Gaaska ammonia waxaa qaboojiya qaboojiyaha si uu u noqdo dareeraha ammonia, ka dibna dareeraha ammonia wuxuu galaa uumiga si uu uumi u baxo, isla markaana wuxuu nuugaa kulaylka dibadda si loo gaaro ujeeddada qaboojinta, sidaas darteed samaynta qaboojinta nuugista faafinta joogtada ah. wareegga.
5. Nitrojiinka waxaa loo isticmaali karaa qaboojiye ahaan xaaladaha "cryogenic", taas oo ah, ku dhawaad 0 darajo (-273.15 darajo Celsius), waxaana guud ahaan loo isticmaalaa shaybaarada si loo barto dabeecadda sare.
6. Daawada, nitrogen dareere ah ayaa caadi ahaan loo isticmaalaa qaboojiye ahaan si loogu sameeyo qalliinka cryoanestesia.
7. Goobta farsamada sare, nitrogen dareeraha ah ayaa badanaa loo isticmaalaa si loo abuuro jawi heerkul hooseeya. Tusaale ahaan, qaar ka mid ah qalabka kor u qaadida waxay helayaan sifooyin awood sare leh oo heerkul hooseeya ka dib marka lagu daaweeyo nitrogen dareere ah.
8. Heerkulka cadaadiska caadiga ah ee nitrogen dareeraha ah waa -196 darajo, kaas oo loo isticmaali karo il qabow heerkul aad u hooseeya. Burburinta heerkulka hooseeya ee taayirada, kaydinta hidda-wadaha isbitaallada, iwm. dhammaan waxay isticmaalaan nitrogen dareere ah il qabow.
2. Sidee bay nitrogen dareere ah u ilaalisaa unugyada?
Farsamada ugu badan ee loo isticmaalo ilaalinta unugyada waa habka loo yaqaan 'cryptopreservation' ee nitrogen dareeraha ah, kaas oo inta badan qaata habka qaboojinta tartiib tartiib ah oo leh qadar habboon oo ah wakiilka ilaalinta si uu u qaboojiyo unugyada.
Fiiro gaar ah: Haddii unugyadu si toos ah u barafoobaan iyada oo aan lagu darin wax difaac ah, biyaha gudaha iyo dibadda unugyada waxay si dhakhso ah u samayn doonaan crystals baraf, taas oo keeni doonta falcelin xun oo isdaba-joog ah. Tusaale ahaan, fuuq-baxa unugyadu waxay kordhiyaan isku-ururinta elektrolytka ee maxalliga ah, waxay beddelaan qiimaha pH, waxayna diidaan borotiinnada qaarkood sababaha kor ku xusan awgeed, taasoo keenaysa qaab-dhismeedka booska gudaha ee unugga inuu khalkhal galo. Waxay keentaa dhaawac, barar mitochondrial, luminta shaqada, iyo khalkhal ku yimaada dheef-shiid kiimikaadka tamarta. Isku-dhafka lipoprotein-ka ee xuubka unugyada sidoo kale si fudud ayaa loo burburiyaa, taasoo keenta isbeddel ku yimaada qulqulka xuubka unugyada iyo luminta waxa ku jira unugyada. Haddii kiristaalo baraf badan laga sameeyo unugyada, marka heerkulka barafku hoos u dhaco, mugga kiristaalo barafka ayaa ballaarin doona, taasoo keentay dhaawac aan laga soo kaban karin oo ku yimaada qaabeynta booska DNA-da nukliyeerka, taasoo keentay dhimashada unugyada.
Kulaylka daahsoon ee macquulka ah ee uu nuugo cuntada nitrogen ee dareeraha ah marka ay cuntadu taabato waxay keentaa in cuntadu baraf noqoto. Nitrojiin dareeraha ah ayaa laga soo saaraa weelka, si lama filaan ah isu beddela heerkulka caadiga ah iyo cadaadiska, oo ka beddelma dareeraha una beddelo xaalad gaas ah. Inta lagu jiro habka isbeddelka wejigan, nitrogen dareeraha ah ayaa karkariyo oo uumi baxa -195.8 ℃ si uu u noqdo nitrogen gaas ah, kulaylka dahsoon ee uumiga waa 199 kJ/kg; Haddii -195.8 Marka heerkulku kor u kaco ilaa -20 °C hoostiisa nitrogen ee cadaadiska atmospheric, waxay nuugi kartaa 183.89 kJ / kg kulayl macquul ah (awoodda kulaylka gaarka ah waxaa loo xisaabiyaa sida 1.05 kJ / (kg? K)), kaas oo la nuugo by by kulaylka uumiga iyo kulaylka macquulka ah ee la nuugo inta lagu jiro habka isbeddelka wajiga nitrogen ee dareeraha ah. Kulaylku wuxuu gaari karaa 383 kJ/kg.
Inta lagu jiro habka qaboojinta cuntada, sababtoo ah xaddi badan oo kuleyl ah ayaa isla markiiba laga qaadaa, heerkulka cuntada si degdeg ah ayaa loo qaboojiyaa dibadda ilaa gudaha si uu u qaboojiyo. Tiknooloojiyada qaboojinta degdega ah ee nitrogen waxay isticmaashaa nitrogen dareere ah isha qabow, taas oo aan waxyeello u lahayn deegaanka. Marka la barbar dhigo qaboojiyaha farsamada dhaqameed, waxay gaari kartaa heerkul hoose iyo heer qaboojin sare. Tiknooloojiyada qaboojinta degdega ah ee nitrogen-ka dareeraha ah waxay leedahay xawaare baraf leh, waqti gaaban, cuntaduna waa mid tayo wanaagsan leh, badbaado sare leh oo aan wasakhayn.
Farsamada qaboojinta degdega ah ee nitrogen-ka dareeraha ah ayaa si weyn loogu isticmaalay qaboojinta degdega ah ee alaabta biyaha sida shrimp, whitebait, carsaanyo bayooloji, iyo abalone. Daraasaduhu waxay muujiyeen in shrimp lagu daweeyay tignoolajiyada qaboojinta degdegga ah ee nitrogen-ka dareeraha ah ay ilaalin karto cusubaanshaha, midabka iyo dhadhanka sare. Taas oo keliya ma aha, bakteeriyada qaar ayaa sidoo kale laga yaabaa in la dilo ama ay joojiyaan taranka heerkulka hooseeya si loo gaaro nadaafad sare Baahida.
Cryopreservation: nitrogen dareere ah waxaa loo isticmaali karaa cryopreservation of noocyo kala duwan oo bayooloji ah, sida unugyo, unug, serum, shahwada, iwm. Shaybaaradan waxaa lagu kaydin karaa muddo dheer heerkulka hoose iyo dib u soo celinta sideedii hore marka loo baahdo. Kaydinta nitrogen ee dareeraha ah waa habka kaydinta inta badan loo isticmaalo, kaas oo inta badan loo isticmaalo cilmi-baarista biomedical, beeraha, xanaanada xoolaha iyo beeraha kale.
Dhaqanka unugyada: nitrogen dareere ah ayaa sidoo kale loo isticmaali karaa dhaqanka unugyada. Inta lagu jiro dhaqanka unugyada, nitrogen dareeraha ah ayaa loo isticmaali karaa in lagu ilaaliyo unugyada hawlgallada tijaabada ah ee xiga. Nitrojiin dareeraha ah ayaa sidoo kale loo isticmaali karaa in lagu qaboojiyo unugyada si loo ilaaliyo jiritaankooda iyo sifooyinka noolaha.
Kaydinta unugyada: Heerkulka hooseeya ee nitrogen dareeraha ah wuxuu ilaalin karaa xasilloonida iyo sharafta unugyada, iyadoo laga hortagayo gabowga unugyada iyo dhimashada. Sidaa darteed, nitrogen dareere ah ayaa si weyn loogu isticmaalaa kaydinta unugyada. Unugyada lagu ilaaliyo nitrogen dareeraha ah si dhakhso ah ayaa loo soo kabsan karaa marka loo baahdo waxaana loo isticmaali karaa wax-is-daba marin tijaabo oo kala duwan.
Codsiga nitrogen dareeraha heerka-cunto waa sida kareemka nitrogen dareeraha ah, buskudka nitrogen dareeraha ah, qaboojinta nitrogen dareeraha ah iyo suuxinta daawada sidoo kale waxay u baahan tahay nitrogen dareere nadiif ah. Warshadaha kale sida warshadaha kiimikada, elektiroonigga, metallurgy, iwm waxay leeyihiin shuruudo kala duwan ee daahirsanaanta nitrogen dareere ah.
Guddiga xisbiga degmada Chuzhou ayaa booqday
ma cabbi kartaa dareere carbon dioxide?