nei nitrogen yemvura inoshandiswa mu cryopreservation?
1. Nei uchifanira kushandisa mvura ine nitrogen sefiriji?
1. Nekuti tembiricha yemvura nitrogenpachayo yakadzikira zvakanyanya, asi hunhu hwayo hwakapfava, uye zvakaoma kuti nitrogen yemvura ipinde mumakemikari, saka inowanzoshandiswa sefiriji.
2.Mvura ine nitrogenvaporizes kutora kupisa, kuderedza tembiricha, uye inogona kushandiswa sefiriji.
3. Kazhinji, ammonia inoshandiswa sefiriji uye mvura semvura.
4. Iyo ammonia gasi inotonhodzwa necondenser kuti ive mvura ammonia, uye ipapo iyo mvura ammonia inopinda muevaporator kuti ibude, uye panguva imwechete inotora kupisa kubva kunze kuti iite chinangwa chefiriji, nekudaro ichigadzira kuenderera kunowedzera kunyura mufiriji. cycle.
5. Nitrogen inogona kushandiswa sefiriji mumamiriro e "cryogenic", kureva, pedyo ne-absolute 0 degrees (-273.15 degrees Celsius), uye inowanzoshandiswa mumarabhoritari kudzidza superconductivity.
6. Mumishonga, nitrogen yemvura inowanzoshandiswa sefiriji kuti iite mabasa pasi pe cryanesthesia.
7. Mumunda wepamusoro-soro, nitrogen yemvura inowanzoshandiswa kugadzira nzvimbo yakaderera-yekushisa. Semuyenzaniso, zvimwe superconducting zvinhu chete kuwana superconducting zvivakwa pazasi tembiricha pashure kurapwa nemvura nitrogen.
8. Tembiricha iri pasi pekumanikidzwa kwakajairika kwemvura ine nitrogen ndeye -196 madhigirii, inogona kushandiswa seyakanyanya-yakaderera tembiricha inotonhora sosi. Iyo yakaderera-yekushisa kupwanya matai, kuchengetwa kwemajini muzvipatara, nezvimwe zvese zvinoshandisa mvura nitrogen sosi inotonhora.
2. Mvura yenitrogen inochengetedza sei masero?
Nzira inonyanya kushandiswa ye cell cryopreservation ndiyo liquid nitrogen cryopreservation method, iyo inonyanya kutora nzira inononoka yechando ine huwandu hwakakodzera hwekudzivirira kuomesa masero.
Ongorora: Kana maseru akaomeswa nechando pasina kuwedzera chero chinhu chinodzivirira, mvura mukati nekunze kwemaseru ichakurumidza kugadzira makristasi echando, izvo zvinokonzeresa zvakatevedzana zvakaipa. Semuyenzaniso, kupera mvura mumuviri kwemaseru kunowedzera nharaunda ye electrolyte, inoshandura pH kukosha, uye denatures mamwe mapuroteni nekuda kwezvikonzero zviri pamusoro, zvichiita kuti mukati memukati mesero ive kusagadzikana. Zvinokonzera kukuvara, mitochondrial kuzvimba, kurasikirwa nebasa, uye kukanganisa kwesimba metabolism. Iyo lipoprotein complex pane sero membrane inoparadzwa zvakare nyore, zvichikonzera shanduko mukupenya kwesero membrane uye kurasikirwa kwesero zviri mukati. Kana mamwe makristasi echando akaumbwa mumasero, sezvo tembiricha yechando ichidzikira, huwandu hwemakristasi echando huchawedzera, zvichikonzera kukanganisa kusingadzoreki kwekugadziriswa kwenzvimbo yenyukireya DNA, zvichikonzera kufa kwesero.
Kupisa kwakadzikama uye kunonzwisisika kunotorwa nemvura yenitrogen chikafu ichisangana nechikafu chinoita kuti chikafu chiome. Liquid nitrogen inoburitswa mumudziyo, inongoerekana yashanduka kuita yakajairika tembiricha uye kudzvanywa, uye inoshanduka kubva kumvura ichienda kune gasi. Munguva yekuchinja kwechikamu ichi, mvura yenitrogen inofashaira uye inobuda pa -195.8 ℃ kuita gaseous nitrogen, uye kupisa kwakadzikama kwekubuda kwemhepo i199 kJ/kg; kana -195.8 Kana tembiricha yakwira kusvika -20 °C pasi penitrogen pamhepo yemhepo, inogona kutora 183.89 kJ/kg yekupisa kunonzwisisika (iyo chaiyo yekupisa inoverengerwa se 1.05 kJ/(kg?K)), iyo inotorwa ne kupisa kwe vaporization uye kupisa kunonzwisisika kunobatwa panguva yemvura nitrogen phase shanduko process. Kupisa kunogona kusvika 383 kJ/kg.
Mukati mekudya kwechando, nokuti huwandu hwakawanda hwekupisa hunotorwa pakarepo, kutonhora kwechikafu kunokurumidza kutonhora kubva kunze kusvika mukati kuti chando. Liquid nitrogen inokurumidza kutonhora tekinoroji inoshandisa mvura nitrogen sosi inotonhora, iyo isina kukuvadza kune nharaunda. Kana ichienzaniswa neyechinyakare mechanical firiji, inogona kuwana yakaderera tembiricha uye yepamusoro kutonhora mwero. Liquid nitrogen inokurumidza kutonhora tekinoroji ine kukurumidza kutonhora, nguva pfupi, uye Chikafu ndechemhando yakanaka, chengetedzo yepamusoro uye isina kusvibiswa.
Liquid nitrogen inokurumidza kutonhora tekinoroji yakashandiswa zvakanyanya mukukurumidza kuomesa nechando yezvigadzirwa zvemumvura zvakaita seshrimp, whitebait, biological crab, uye abalone. Zvidzidzo zvakaratidza kuti shrimp inobatwa nemvura nitrogen inokurumidza kutonhora tekinoroji inogona kuchengetedza kutsva kwakanyanya, ruvara uye kuravira. Kwete izvozvo chete, mamwe mabhakitiriya anogona zvakare kuurayiwa kana kumisa kubereka pakadzika tembiricha kuti awane hutsanana hwepamusoro hunodiwa.
Cryopreservation: Liquid nitrogen inogona kushandiswa cryopreservation yemhando dzakasiyana-siyana dzezvipenyu, semasero, matishu, serum, urume, etc. Aya masampuli anogona kuchengetedzwa kwenguva yakareba pakudziya kwakaderera uye kudzoserwa kumamiriro awo epakutanga kana zvichidikanwa. Liquid nitrogen cryopreservation inowanzoshandiswa kuchengetedza nzira, iyo inowanzoshandiswa mukutsvagisa biomedical, kurima, kupfuya mhuka nemimwe minda.
Sero tsika: Liquid nitrogen inogona zvakare kushandiswa sero tsika. Munguva yesero tsika, mvura nitrogen inogona kushandiswa kuchengetedza maseru kune anotevera ekuedza mashandiro. Liquid nitrogen inogona zvakare kushandiswa kuomesa masero kuchengetedza kugona kwavo uye zvipenyu.
Kuchengetera maseru: Tembiricha yakaderera yenitrogen yemvura inogona kuchengetedza kugadzikana uye kuvimbika kwemaseru, uku ichidzivirira kukwegura kwesero uye kufa. Naizvozvo, mvura nitrogen inoshandiswa zvakanyanya mukuchengetedza masero. Masero akachengetwa munitrogen yemvura anogona kudzoserwa nekukurumidza pazvinenge zvichidiwa uye oshandiswa pakuedza kwakasiyana siyana.
Kushandiswa kwechikafu-giredhi yemvura nitrogen yakaita semvura nitrogen aizikirimu, liquid nitrogen mabhisikiti, liquid nitrogen kutonhora uye anesthesia mumishonga zvinodawo yakakwirira-kuchena mvura nitrogen. Mamwe maindasitiri akadai seindasitiri yemakemikari, zvemagetsi, simbi, nezvimwe zvine zvinodiwa zvakasiyana pakuchena kwenitrogen yemvura.
