Vim li cas cov kua nitrogen siv hauv cryopreservation?
1. Vim li cas thiaj siv kua nitrogen ua cov tub yees?
1. Vim qhov kub ntawmkua nitrogennws tus kheej yog qhov tsawg heev, tab sis nws qhov xwm txheej yog me me, thiab nws yog qhov nyuaj rau cov kua nitrogen kom tsis txhob muaj tshuaj lom neeg, yog li nws feem ntau siv los ua cov tub yees.
2.Cov kua nitrogenvaporizes kom nqus cua sov, txo qhov kub thiab siv tau ua cov tub yees.
3. Feem ntau, ammonia yog siv los ua refrigerant thiab dej li absorbent.
4. Cov roj ammonia yog txias los ntawm lub condenser los ua kua ammonia, thiab tom qab ntawd cov kua ammonia nkag mus rau hauv lub evaporator kom evaporate, thiab tib lub sij hawm absorbs cua sov los ntawm sab nraud kom ua tiav lub hom phiaj ntawm tub yees, yog li tsim ib tug nruam diffusion haum tub yees. lub voj voog.
5. Nitrogen tuaj yeem siv los ua cov tub yees nyob rau hauv "cryogenic" tej yam kev mob, uas yog, ze rau qhov tseeb 0 degrees (-273.15 degrees Celsius), thiab feem ntau yog siv nyob rau hauv lub chaw soj nstuam los kawm superconductivity.
6. Hauv cov tshuaj, cov kua nitrogen feem ntau yog siv los ua cov tub yees ua haujlwm raws li cryoanesthesia.
7. Nyob rau hauv high-tech teb, kua nitrogen feem ntau yog siv los tsim ib qho chaw uas tsis kub. Piv txwv li, qee cov khoom siv superconducting tsuas yog tau txais cov khoom siv superconducting ntawm qhov kub thiab txias tom qab tau kho nrog kua nitrogen.
8. Qhov kub thiab txias nyob rau hauv ib txwm siab ntawm cov kua nitrogen yog -196 degrees, uas yuav siv tau raws li ib tug ultra-tsawg kub txias qhov chaw. Qhov ntsuas kub qis ntawm cov log tsheb, cov noob khaws cia hauv tsev kho mob, thiab lwm yam, txhua tus siv cov kua nitrogen ua kom txias.
2. Cov kua nitrogen khaws cov hlwb li cas?
Cov txheej txheem siv ntau tshaj plaws rau kev khaws cia ntawm tes yog cov kua nitrogen cryopreservation txoj kev, uas feem ntau yog siv cov txheej txheem qeeb qeeb nrog qhov tsim nyog ntawm tus neeg tiv thaiv kom khov hlwb.
Lus Cim: Yog tias cov cell ncaj qha khov yam tsis tau ntxiv cov tshuaj tiv thaiv, cov dej hauv thiab sab nraud ntawm lub hlwb yuav sai sai ua cov dej khov, uas yuav ua rau muaj kev cuam tshuam tsis zoo. Piv txwv li, lub cev qhuav dej ntawm cov hlwb ua rau lub zos electrolyte concentration, hloov pH tus nqi, thiab denatures ib co proteins vim hais tias saum toj no yog vim li cas, ua rau lub internal chaw ntawm lub cell tsis zoo. Ua rau kev puas tsuaj, mitochondrial o, tsis ua haujlwm, thiab cuam tshuam ntawm lub zog metabolism. Cov lipoprotein complex ntawm cov cell membrane kuj yooj yim puas, ua rau kev hloov hauv permeability ntawm cell membrane thiab poob ntawm cov ntsiab lus ntawm tes. Yog hais tias muaj ntau cov dej khov nab kuab tsim nyob rau hauv lub hlwb, raws li lub freezing kub txo, lub ntim ntawm cov dej khov muaju yuav nthuav, uas ua rau irreversible kev puas tsuaj rau lub spatial configuration ntawm nuclear DNA, ua rau cell tuag.
Lub latent thiab paub qab hau kub absorbed los ntawm cov kua nitrogen cov zaub mov nyob rau hauv kev sib cuag nrog cov zaub mov ua rau cov zaub mov khov. Cov kua nitrogen tawm ntawm lub thawv, dheev hloov mus rau qhov kub thiab txias, thiab hloov los ntawm cov kua mus rau lub xeev gaseous. Thaum lub sij hawm no cov txheej txheem hloov, cov kua nitrogen boils thiab evaporates ntawm -195.8 ℃ los ua gaseous nitrogen, thiab latent kub ntawm evaporation yog 199 kJ / kg; Yog tias -195.8 Thaum qhov kub nce mus txog -20 ° C hauv qab nitrogen ntawm atmospheric siab, nws tuaj yeem nqus tau 183.89 kJ / kg ntawm qhov ntsuas kub (qhov tshwj xeeb kub muaj peev xwm suav tau li 1.05 kJ / (kg? K)), uas yog absorbed los ntawm cov cua sov ntawm vaporization thiab paub qhov cua kub absorbed thaum lub sij hawm ua kua nitrogen theem hloov txheej txheem. Cov cua sov tuaj yeem ncav cuag 383 kJ / kg.
Nyob rau hauv tus txheej txheem ntawm cov khoom noj khov, vim hais tias ib tug loj npaum li cas ntawm tshav kub yog muab tshem tawm nyob rau hauv ib tug instant, qhov kub ntawm cov zaub mov yog sai txias los ntawm sab nraud mus rau sab hauv kom khov. Cov kua nitrogen ceev-freezing tshuab siv cov kua nitrogen ua cov dej txias, uas tsis muaj kev puas tsuaj rau ib puag ncig. Piv nrog rau cov neeg kho tshuab tub yees, nws tuaj yeem ua tiav qhov kub thiab txias dua. Cov kua nitrogen ceev-freezing thev naus laus zis muaj lub zog khov nrawm, lub sijhawm luv, thiab cov khoom noj khoom haus zoo, muaj kev nyab xeeb thiab tsis muaj kuab lom.
Cov kua nitrogen ceev-freezing tshuab tau dav siv nyob rau hauv ceev-freezing ntawm cov khoom dej xws li cws, whitebait, lom roob ris, thiab abalone. Cov kev tshawb fawb tau pom tias cw kho los ntawm cov kua nitrogen sai-freezing thev naus laus zis tuaj yeem tswj tau qhov tshiab, xim thiab saj. Tsis tas li ntawd xwb, qee cov kab mob kuj tseem raug tua los yog tso tseg kev tsim tawm ntawm qhov kub thiab txias kom ua tiav kev huv huv dua.
Cryopreservation: Cov kua nitrogen tuaj yeem siv rau cryopreservation ntawm ntau yam kev kuaj lom, xws li cov hlwb, cov ntaub so ntswg, ntshav, phev, thiab lwm yam. Cov qauv no tuaj yeem khaws cia rau lub sijhawm ntev ntawm qhov kub thiab txias thiab rov qab los rau lawv qhov qub thaum xav tau. Cov kua nitrogen cryopreservation yog ib txoj hauv kev khaws cia, uas feem ntau siv rau hauv kev tshawb fawb txog biomedical, kev ua liaj ua teb, tsiaj txhu thiab lwm yam liaj teb.
Cell kab lis kev cai: Kua nitrogen kuj siv tau rau cell kab lis kev cai. Thaum lub sij hawm kab lis kev cai ntawm tes, kua nitrogen tuaj yeem siv los khaws cov hlwb rau kev sim tom ntej. Cov kua nitrogen kuj tuaj yeem siv los khov cov hlwb kom khaws lawv cov txiaj ntsig thiab cov khoom siv lom neeg.
Cell cia: Qhov kub ntawm cov kua nitrogen tsis tshua muaj peev xwm tswj tau qhov ruaj khov thiab kev ncaj ncees ntawm cov hlwb, thaum tiv thaiv cell aging thiab tuag. Yog li ntawd, kua nitrogen yog dav siv nyob rau hauv cell cia. Cells khaws cia hauv cov kua nitrogen tuaj yeem rov qab tau sai thaum xav tau thiab siv rau ntau yam kev sim ua haujlwm.
Daim ntawv thov ntawm cov zaub mov-qib kua nitrogen yog zoo li kua nitrogen ice cream, kua nitrogen biscuits, kua nitrogen freezing thiab tshuaj loog nyob rau hauv cov tshuaj kuj yuav tsum tau high-purity kua nitrogen. Lwm cov kev lag luam xws li tshuaj lom neeg kev lag luam, hluav taws xob, metallurgy, thiab lwm yam. muaj cov kev cai sib txawv rau cov purity ntawm kua nitrogen.
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